Table of Contents
GeneMANIA finds other genes that are related to a set of input genes, using a very large set of functional association data. Association data include protein and genetic interactions, pathways, co-expression, co-localization and protein domain similarity. You can use GeneMANIA to find new members of a pathway or complex, find additional genes you may have missed in your screen or find new genes with a specific function, such as protein kinases. Your question is defined by the set of genes you input.
Find more genes like these
Tell me about my gene(s)
Predicted related genes can be, for instance, in the same pathway or complex as your input genes, can be co-expressed or have similar enzymatic function. To determine how predicted genes are related to your input genes, you need to study the links in the network to find out how your input genes are connected to each other and how new genes are related to your input genes.
GeneMANIA has the advantage of flexibility, accuracy, and often speed of response over these other systems. In particular, in a competition (on yeast (Mostafavi et al., 2008 PDF, PubMed, journal) and mouse (Pena-Castillo et al., 2008 PDF, PubMed, journal), GeneMANIA was shown to be more accurate than other gene function prediction methods, and is generally faster, producing predictions within seconds. Because of this speed, GeneMANIA can produce results while you wait. Users can select arbitrary subsets of networks that they want to query and GeneMANIA automatically selects network weights based on the input gene list, generating a network specific to the user’s gene list. Unlike other systems, GeneMANIA provides users with the ability to upload their own network and also compensates for redundancies in the data, so users don’t have to worry about double-counting interactions.
GeneMANIA stands for Multiple Association Network Integration Algorithm.
The GeneMANIA algorithm consists of two parts:
GeneMANIA treats gene function prediction as a binary classification problem. As such, each functional association network derived from the data sources is assigned a positive weight, reflecting the data sources’ usefulness in predicting the function. The weighted average of the association networks is constructed into a function-specific association network. GeneMANIA uses separate objective functions to fit the weights; this simplifies the optimization problem and decreases the run time.
GeneMANIA predicts gene function from the composite network using a variation of the Gaussian field label propagation algorithm that is appropriate for gene function prediction in which there are typically relatively few positive examples. Label propagation algorithms assign a score (the discriminant value) to each node in the network. This score reflects the computed strength of association that the node has to the seed list defining the given function. This value can be thresholded to enable predictions of a given gene function.
GeneMANIA: a real-time multiple association network integration algorithm for predicting gene function
Mostafavi S, Ray D, Warde-Farley D, Grouios C, Morris Q (2008)
[Genome Biology 9: S4.
GeneMANIA recognizes Entrez, Ensembl, Standard gene symbols, Uniprot/SwissProt and RefSeq identifiers and unique gene names.
The process used to create interaction networks from source data for GeneMANIA is described on the data processing page. This same process is used both to produce the core networks included with GeneMANIA, as well as for networks created from user-uploaded data at the GeneMANIA Website or using the Cytoscape Plugin.
The interaction networks used in the current GeneMANIA website, as well as archived datasets from previous releases are available for download as plain text files. The data archive page describes the file formats used.
We recommend citing the NAR webserver issue, as follows.
The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function
Warde-Farley D, Donaldson SL, Comes O, Zuberi K, Badrawi R, Chao P, Franz M, Grouios C, Kazi F, Lopes CT, Maitland A, Mostafavi S, Montojo J, Shao Q, Wright G, Bader GD, Morris Q
See the instructions.
That version has been superseded by an improved interface that does not use Flash. The old version is available at old.genemania.org.